Using structure-guided design, pyrene maleimide was attached to pairs of Cys residues separated by ~5 Å increments on helix 2 of the N-terminal domain of apolipoprotein E3 (apoE3). The fluorescence and excitation spectra of pyrene at high concentrations were studied along the sol to xerogel stages of the sol–gel silica formed by the acidic hydrolysis of tetraethyl orthosilicate. The systematic description of the complex photophysical behaviour of pyrene in surfactant solutions in combination with a quantitative model for the surfactant concentrations reproduces with high accuracy the steady-state and the time resolved fluorescence intensity of pyrene in surfactant solutions near the cmc, both in the monomer and in the excimer emission bands. To improve the fluorescence properties of pyrene, we have designed a pyrene-2,7-diyl bridged macrocage in which the pyrene moiety is sterically protected by the outside alkyl chains. This process is experimental and the keywords may be updated as the learning algorithm improves. The ratio of intensity of excimer to monomer emission was measured on solutions of pyrene in N,N-dimethylformamide (DMF) at various temperatures from the decay curves of excimer emission due to pulse excitation with a nitrogen laser. A probe of sulfhydryl proximity and local chain separation. The macrocage shows intense fluorescence from a monomeric excited state without excimer fluorescence even in saturated solutions, although the parent pyrene shows excimer fluorescence in highly concentrated … pyrene fluorescence emission can bequenched due to the diffusion controlled s with a formation of excimer characteristic emission band at longer wavelengths around 500 nm [24,40,46-49]. The time dependence of the pyrene fluorescence intensity after pulsed excitation follows Stern-Volmer quenching kinetics in pure water but has a much Rabbit skeletal alphaalphatropomyosin was specificially labeled at cysteine 190 with the fluorescent reagent, N-(1-pyrene)maleimide. Pyrene–HumAfFt bioconjugate, thanks to the peculiar pyrene excimer properties, helped unravelling the kinetic details of the archaeal ferritins oligomerization process, by means of an easily accessible spectroscopic technique and, at the same time, allowed the visualization by two photon fluorescence microscopy of HumAfFt uptake by HeLa cells. Therefore, two excimer processes can be identified by the excitation spectra. Temperature effect in electrochemiluminescenece (ECL) of pyrene was investigated in order to clarify the formation of excimers. Fluorescence spectral analysis revealed an intense excimer band when the probes were ~5 Å from each other with an e/m ratio of ~3.0, which decreased to ~1.0 at 20 Å. Pyrene excimer fluorescence in rabbit skeletal alphaalphatropomyosin labeled with N-(1-pyrene)maleimide. Pyrene Ring Pyrene Derivative Excimer Formation Monomer Fluorescence Excimer Fluorescence These keywords were added by machine and not by the authors. The monomer and excimer fluorescence of pyrene were observed in the reaction system. Betcher-Lange SL, Lehrer SS.
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